SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. FlexStation™ I. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. 3 or greater. 312 gain calibration failed. Tecan Infinite 200 PRO M Plex Multimode Microplate Reader 30050303. . Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. FI assay. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forIntroduction. 과 같은 Parameter를 검사하여 평가할 수 있습니다. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. This user guide provides detailed instructions on how to perform various tests, interpret the results, and troubleshoot common issues. 314 low lamp level warning. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellLuminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. Actual shake speed is based on the microplate format. Male 8 pin mini Din to Female 9D. In the M2, M5 and Plus 384, the light. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. book Page 21 Wednesday, July 7, 2010 2:09 PM. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5,. Recommended SpectraMax iD3/iD5 reader settings. 0 mm hex key. At each time point, cells were incubated with CCK-8 reagent for 1 hour at 37 °C, followed by measuring the optical absorbance at 450 nm with the SpectraMax M3 microplate reader (Molecular Devices. book Page 1 Wednesday, July 7, 2010 2:09 PM Plate chamber design of the SpectraMax category 1 microplate readers. Fluorescence was quantified on SpectraMax M3 plate reader, Ex 485/Em 520 using SoftMax Pro software. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. 3: The cuvette chamber. User Manual – SpectraMax M3 M4 M5 M5e. 316 grating motor driver faulty. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. The actual pathlength, d, of a solvent is found from the following equation: d cm. 7. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The mean fluorescence intensity (MFI) was detected with a. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Turn the instrument around so that the back of the instrument is. SpectraMax Microplate Readers. The optical density (OD) was read by SpectraMax M3 (Molecular Devices, Sunnyvale, CA). Molecular Devices Spectramax Plate Reader. 37) was ordered from Sigma Aldrich (St. Add to Cart. . The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. Bioz Stars score: 86/100, based on 1 PubMed citations. To avoid injury, it is recommended that two. % EPS by weight was calculated by standardization of each mean concentration of proteins, carbohydrates, and eDNA to μg/ml, then divided by total starting weight of pooled crude EPS from ten samples. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The shift in emission peak is also automaticallyThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Plate Formats: 6, 12, 24, 48, 96, 384 wells. The Challenge. Align the two slots on the bottle holder with the two pegs on the rail. Our absorbance plate readers feature our PathCheck Sensor. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. 0 out of 5 $ 11,999. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. Features. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 2. The team have compared the effects of antibiotics on bacterial viability and. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-pletely automated when used with SoftMax ® Pro software. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. book Page 1 Wednesday, July 7, 2010 2:09 PM. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Bioz Stars score: 86/100, based on 1 PubMed citations. 2. Validate the optical performance of your absorbance . The ROS level was detected by a reactive oxygen species assay kit (Beyotime, China). Introduction. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). com. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Make sure that the microplate reader feet are completely seated in the mounting cups. Type: Clear: Add to Cart. To determine growth rate, the log phase of growth. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Spectramax PMT voltage? I used spectramax M3 plate reader to measure the fluorescence change through time course. 1 & 7. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The bacterial colony was mixed with. 0 out of 5 $ 12,499. SpectraMax® Paradigm® Multi-Mode Detection Platform. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Generate more data points without the need to dilute. Shake intensity. Total phenolic contents were calculated from the gallic acid standard curve. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. 6: bioNova científica, s. 115 SpectraMax M4 Multi-Mode Microplate Reader. 005) Photometric PrecisionSpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. Lift the right side of the microplate reader and place the feet into the two mounting cups on the baseplate. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for If application. Wavelength precision. com. Next to the pushbutton is a green LED. For example, microplates containing different samp les can be read using the same or We would like to show you a description here but the site won’t allow us. Loosen the captive screws on the front of the instrument and then raise the top cover up and over the instrument. D 1 1. Learn more about its features, specifications, and applications in this user. 0 OD for earlier models. Wavelength Selection. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 4. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The chamber contains springs that position the cuvette in the proper alignment for a read. Impact of fix:Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. M2. We would like to show you a description here but the site won’t allow us. Other technologies such as imaging. Description 1. Lower limits of detection for ATP determined using the ATPlite . After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform. The chamber. Page 26 The trademarks used herein are the. SpectraMaxi3Multi. With optimized reagents, option to operate the. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Expanded dynamic range. For more sample throughput, the system can StakMax Handling Stacker Base Unit. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. SpectraMax M5/M5e/M4/M3/M2/M2e. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. For SpectraMax L or LMax II, LMax II. 1). The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). NETosis Assays Using Immunostaining and Microscopy. 2. . 9000-0756. Table 1. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). 3 software. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Spectral scans were performed on native and denatured lysozyme samples using the SpectraMax iD3 reader with excitation at 270 nm and emission ranging from 300 nm to 450 nm. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 2. . SoftMaxProSoftwareUserGuide 2 5014177G ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevices,LLC(“Molecular Devices”)equipment,software,reagents. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. ROS detection. These lab workhorses are used in drug discovery, research, bioassay validation, and. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Page 22 StakMax Microplate Handling System User Guide 3. Add to Cart. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. SpectraMax Mini. WARNING! The instrument weighs approximately 36 pounds (16. Examples: “Press carefully to engage,” “ Do not press down. 2. Qualify the fluorescence performance of the SpectraMax . no. Other technologies such as imaging. item 6 White 18-SMD LED License Plate Lights For 04-06 BMW E46 LCI 325Ci 330Ci M3 Coupe White 18-SMD LED License. 2. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. no. SpectraMax i3 Multi-Mode Platform A flexible, user-upgradeable microplate detection system Key Features The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. We would like to show you a description here but the site won’t allow us. Cables. 1. 304 signal level saturation. This process should take about 3 - 5 minutes. For all. 0. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Multi-Mode Microplate Readers. Using. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. SpectraMax® ABS. Antiretroviral efficacy was determined by measuring HIV reverse transcriptase (RT) activity in culture medium. All configurations. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. The ALP activity level was determined, relative to that of the control group, as. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. SpectraMax ABS Plus SpectraTest ABS2 v1. I used the same plate and set the PMT to be automatic. Carefully place the cytometer on its side on the foam pad so that the top of the cytometer faces the bottom of the microplate reader. Resolution: The validation protocols are included in the v5. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. e. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 2 release. 1) EarlyTox Glutathione Assay (6. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. book Page 1 Tuesday,. 2 only) SpectraMax M-series Microplate readers. 01) + 0. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). . Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Background signal was determined by adding 500 U/ml Catalase from Bovine Liver (Sigma-Aldrich, E3289) in one well to convert hydrogen peroxide into. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. Category 2 and 3 microplate readers use gradient heating as well. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Choose from a three- (M3), four- (M4), orThe “M3 M4 M5 M5e ABS1” and “M3 M4 M5 M5e FL1” validation protocols are not included in v5. No accessories are needed and SoftMax® Pro. Microplate Readers Biotek Synergy 2 Plate. 7°C and the bottom to 36. 1) SpectraMax Glo Steady Luc Reporter Assay (6. Description 1. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. SpectraMax DuoLuc Reporter Assay (SMP 6. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Cables. Capture Flash Assays with Ease. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Wait 20 seconds. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Molecular Devices SpectraMax® series microplate readers. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Note: In this user guide, SpectraMax ® refers to several SpectraMax . 2. Example: [Drawer]. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Multimode Readers Comparison Chart. SpectraMax ® M2 Multi-Mode Microplate Reader. Then, the absorbance was measured using a spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at 734 nm. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. I am looking to meet/connect with people from all walks of life. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. Antiretroviral activities. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:The bound CV was dissolved in 30% acetic acid and the absorption was measured in a microplate reader at OD 590 using SpectraMax M3 (Molecular Devices, United States). SpectraMax® M5e. 0 out of 5 $ 2,999. {"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. StakMax. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. 3 software (Molecular Devices, San Jose, CA, USA). Data for Spectramax M3 Spectrophotometer gathered from related PubMed articles. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. a SpectraMax M3 microplate reader (Molecular Devices Inc. SoftMax Pro. Detection modalities are shown in Table 1-1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 306 plate air cal. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The drawing shows a standard microplate of 15 mm height covered with a lid. Choose from a three- (M3), four- (M4), or SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Multimode Readers Comparison Chart. Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract. STAKMAX. SpectraMax® M4. Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The corrected absorbance is within 5% of the. Expanded wavelength coverage. Click SoftMax Pro Software User Guide. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. ZERO BIAS - scores, article reviews, protocol conditions and moreF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Learn more about its features, specifications, and applications in this user guide. Published Dec 16, 2020 | Updated Dec 16, 2020. SoftMax Pro GxP is a special software release that includes full user and permission con-SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 1. Light Source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Description 1. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 116 SpectraMax M5 and M5e Multi-Mode. Incubate at 37ºC for 1–4 hours. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Add to Cart. Select wells and then add or select a group or blank and assignThe SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. . Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. spr SpectraMax iD3 SpectraTest ABS2 v1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The information below applies to a standard 96 well plate, set to a linear shake mode. Figure 2-23: Bottle holder To install the bottle holder: 1. The exactly same condition without CCK-8 solution was used to subtract background signal that is absorbed by cell medium or natural product. The SoftMax® Touch Software is installed in. 1. standard features include spectral scanning across the wavelength range in 1 nm increments, up to six absorbance wavelengths per read, and up to four wavelength pairs for other read modes. Serum IL-8 level quantification Serum IL-8 levels were quantified in sera from patients after HSCT using an enzyme-linked immunosorbent assay (Cat. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. Fundamentally, one is interested in whether or not an instrument can detect a signal for a given assay; that is, whether a signal is detectable above the background of the instrument. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentThe SpectraMax iD3 reader offers the sensitivity and wavelength scanning capability needed to perform intrinsic tryptophan fluorescence measurements. Unparalleled performance on a personalized platform. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. D 47 7. Absorbance was read at 570 nm using a SpectraMax M3. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. Photometric accuracy. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. ISM Equipment Classification (Group 1, Class A) This equipment is. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. A. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 1) Select the correct plate and the wells that you want to measure. 7. , contact Molecular. 3: The cuvette chamber. 2. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. For each extract concentration, percentage parasite viability and. The % parasite viability, as indicated by the pLDH activity in the treated wells compared with the untreated controls, was calculated. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Microplate Readers Molecular Devices VersaMax Microplate Reader. 00 .